The logistic of this article
build a complete transection model (this model still have the capability to regenerate), and this model could be used to assess the axonal regeneration.
1. Expression of Sema3A and other axonal growth inhibitors:
(1) Analyzed the spatiotemporal expression of Sema3A protein in the transected spinal cord.
(2) Two weeks after transection injury, observe the fibronectin-positive scar tissue, to see the lesion epicenter where Sema3A protein expression was also prominently detected. That is, where the scar tissue present, where the Sema3A there is.
(3) In situ hybridization